Study of infectious diseases in archaeological bone material – A dataset
نویسندگان
چکیده
Bones of human and ground sloth remains were analyzed for presence of Trypanosoma cruzi by conventional PCR using primers TC, TC1 and TC2. Sequence results amplified a fragment with the same product size as the primers (300 and 350pb). Amplified PCR product was sequenced and analyzed on GenBank, using Blast. Although these sequences did not match with these parasites they showed high amplification with species of bacteria. This article presents the methodology used and the alignment of the sequences. The display of this dataset will allow further analysis of our results and discussion presented in the manuscript "Finding the unexpected: a critical view on molecular diagnosis of infectious diseases in archaeological samples" (Pucu et al. 2017) [1].
منابع مشابه
The value of Kinesiography in the Diagnosis of Bone and Joint Diseases in Pediatric Medicine
Bone scan in diagnosis of infectious osteoarthritis Bone scan with Technezium 99m is harmless methodoi evaluation of Skeletal lesions.It is safe 1n pedia_ trics age group and it can be used in early diffe-rentiate osteomyelitis from cellulits sand also it may help in diagnosis of subsclinical involvement of eumatoid arthritis,benign and malignant bone tumors stress fractures and periostit...
متن کاملEvaluating bacterial pathogen DNA preservation in museum osteological collections.
Reports of bacterial pathogen DNA sequences obtained from archaeological bone specimens raise the possibility of greatly improving our understanding of the history of infectious diseases. However, the survival of pathogen DNA over long time periods is poorly characterized, and scepticism remains about the reliability of these data. In order to explore the survival of bacterial pathogen DNA in b...
متن کاملEvaluation of appropriate antibiotic administration by general practitioners at the emergency department of a teaching hospital in Sanandaj, Iran
Background and Aim: In recent decades, the emergence of resistant strains of staphylococcus aureus, pneumococci, enterococcus and intestinal gram-negative bacteria due to the inappropriate use of antibiotics has emphasized the necessity of proper use of antibiotics. The aim of this study was to evaluate the appropriate antibiotic administration by general practitioner in the emergency departmen...
متن کاملBone collagen preservation in the tropics: a case study from ancient Puerto Rico
Bone collagen is a well-characterized and generally robust protein that plays a key role in both radiometric dating and stable isotopic reconstruction of paleodiet. To be useful for such purposes, however, analyzed collagen must be relatively taphonomically unaltered. To date, little research has been conducted to document the taphonomic fate of bone collagen from archaeological sites in the tr...
متن کاملGilan Iron Age Diet: Results based on chemical analysis on samples of human and animal bones
Trace element analyses was carried out using Atomic Absorption Spectrophotometer and XRF on a small collection of bones and teeth from horse and human bone samples to check the ratio of strontium and calcium and other elements like zinc and copper. Interestingly the copper representation in humans is much higher compared to that of Sr/Ca. This shows that their diet consisted mainly of animal me...
متن کامل